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US 4,740,461 AGranted
Vectors and methods for transformation of eucaryotic cells
AssigneeGenetics Institute, Inc.
InventorRandal J. Kaufman
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Abstract
Eucaryotic cells cotransformed with product and selection genes yield considerably greater quantities of product after a novel subcloning strategy is employed: Transformants are identified for product yield, cultured under selection pressure and the progeny screened for product yield. Novel transformation vectors contain directly ligated selection and product genes and/or eucaryotic promoters.
Claims12
In a method for transforming eukaryotic cells to incorporate a desired product gene by the technique of cotransfecting with unlinked vectors, one of which contains said product gene, the improvement comprising:
- cotransfecting the cells with
- (a) a first vector comprising an enhancer, a first promoter, and a product gene under the transciptional control of the first promoter, said first vector not containing a selection gene under the control of the first promoter;
- and,
- (b) a second unlinked vector comprising a selection gene under the transciptional control of a second promoter dependant on the enhancer, the second vector not containing the enhancer.
1 more element
Dependencies are inferred from the claim text.