Loading patent…
AssigneeEli Lilly and Company
InventorsRamamoorthy Belagaje, Janet K. Epp, JoAnn Hoskins, Hansen M. Hsiung, George L. Long, Brigitte E. Schoner
Estimating the term…
The present invention comprises improved derivatives of the tryptophan operon useful for expressing fused gene products in E. coli and relate organism. Two of the improved derivatives disclosed are encoded on 0.43 and 0.55 kb EcoRI restriction fragments from plasmids pCZ20 and pLEBGH2 (in strains NRRL B-15881 and NRRL B-15882), respectively. The modified derivatives have been placed on recombinant DNA cloning and expression vectors. A variety of expression vectors have been constructed that drive expression of fused gene products. Two novel gene sequences, encoding insulin-like growth factors I and II, have been expressed with the modified tryptophan operon system.
In the derivative of the tryptophan operon promoter-operator-leader sequence wherein said sequence comprises a transcriptional activating sequence, a transitional activating sequence and a DNA coding region for a fused leader-trpE gene sequence from which the sequence encoding the carboxy terminus of the leader peptide, the attenuator region of the leader sequence and the proximal region of the trpE gene have been deleted, an improvement wherein the improvement comprises a shortened LE-encoding sequence, said shortened LE-encoding sequence being contained within a trpLE sequence comprising about 428 to about 548 deoxyribonucleotide pairs.
The shortened trpLE-encoding sequence of calim 1 which is about 428 deoxyribonucleotide pairs.
Dependencies are inferred from the claim text.