Aminopyrazolinones, reagent containing them and the use thereof in the enzymatic determinaton of hydrogen peroxide
AssigneeBoehringer Mannheim GmbH
InventorsHans-Georg Batz, Rupert Herrmann, Joachim Siedel
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Abstract
The present invention provides aminopyrazolinones of the general formula: ##STR1## wherein R.sup.1, R.sup.2 and R.sup.3, which can be the same or different, are straight-chained or branched alkyl radicals containing up to 6 carbon atoms and together containing at most 8 carbon atoms. The present invention also provides a process for the preparation of these new aminopyrazolinones and reagents containing them. In addition, the present invention is concerned with the use of these new aminopyrazolinones as a color coupler for the detection of hydrogen peroxide.
Claims9
An aminopyrazolinone of the formula:
- ##STR5## wherein R.sub.1 is C.sub.1 -C.sub.6 alkyl,
- R.sub.2 is C.sub.1 -C.sub.6 alkyl and
- R.sub.3 is C.sub.1 -C.sub.6 alkyl,
- with the proviso that R.sup.1, R.sup.2 and R.sup.3 together total 3 to 8 carbon atoms.
4-Amino-1,2,3-trimethylpyrazolin-5-one.
In a method of detection of hydrogen peroxide the improvement wherein, an aminopyrazolinone of the formula:
- ##STR6## wherein R.sub.1 is C.sub.1 -C.sub.6 alkyl, R.sub.2 is C.sub.1 -C.sub.6 alkyl and R.sub.3 is C.sub.1 -C.sub.6 alkyl, with the proviso that R.sup.1, R.sup.2 and R.sup.3 together total 3 to 8 carbon atoms, is used as a colour coupler for the detection of hydrogen peroxide in the presence of peroxidase, with a phenolic or anilinic coupling component.
Dependencies are inferred from the claim text.