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US 4,735,901 AGranted
Transformation of Candida albicans
AssigneeE. R. Squibb & Sons, Inc.
InventorsMyra B. Kurtz, Donald R. Kirsch
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Abstract
Candida albicans has been transformed by the insertion of a plasmid comprising a fragment of DNA from Candida albicans containing the intact ADE 2 gene.
Claims7
A plasmid comprising a fragment of DNA from Candida albicans containing the intact ADE 2 gene.
Candida albicans, having inserted therein a plasmid comprising a fragment of DNA from Candida albicans containing the intact ADE 2 gene.
A method of introducing exogenous DNA into Candida albicans comprising:
- (i) enzymatically removing the cell wall of Candida albicans;
- (ii) adding exogenous DNA to the resultant Candida albicans spheroplasts;
- (iii) fusing the exogenous DNA with the Candida albicans;
- and
- (iv) selecting the Candida albicans cells having the exogenous DNA incorporated therein.
2 more elements
Dependencies are inferred from the claim text.