Monoclonal antibodies and method of identifying species using the same
AssigneeThe University of Virginia Alumini Patents Foundation
InventorsJohn C. Herr, David C. Benjamin
Life of this patent
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Abstract
The new method an products recognize human serum albumin, differentiating blood and tissue from human sources from blood and tissue of other animals. The method and products are particularly useful in forensic investigations. First, mice are injected with purified human serum albumin, spleen cells from the mice are fused with cells from a murine myeloma. Resulting hyberdomas are screened on human serum albumin coated plates. Subcloning, stabilizing by culture and continued assays for anti-human albumin activity produces products which recognize and differentiate blood or tissue samples from samples of other species.
Claims11
Hybrid cell line ATCC No. 8860 secreting monoclonal antibodies specifically reactive with human serum albumin.
A method of identification of species of origin of blood, bloodstain, or tissue sample comprising contacting a sample containing albumin with anti-albumin monoclonal antibodies specific to a particular species and sensing reactivity of the monoclonal antibody with the albumin from the sample.
A forensic kit for identification of human species of blood, bloodstain, or tissue samples comprising human anti-albumin monoclonal antibodies, plate means for containing the monoclonal antibodies with albumin from a sample, enzyme and substrate for indicating the reactivity of the monoclonal antibodies with albumin from the sample.
Dependencies are inferred from the claim text.