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US 4,735,794 AGranted
Method for drying biological specimens
AssigneeTed Pella, Inc.
InventorMartin C. Parkinson
Life of this patent
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Abstract
A method for preparing biological specimens for the scanning electron microscope utilizes a select group of fluorocarbon solvents. Replacement of drying solvents with a fluorocarbon solvent, followed by direct sublimation, provides comparable results to critical point drying.
Claims5
A method for preparing a biological specimen for examination in the scanning electron microscope, which comprises the steps of:
- (A) Drying said specimen in a graded series of a drying solvent;
- (B) Replacing said drying solvent within said specimen by immersing said specimen in a melted fluorocarbon solvent selected from the group consisting of
- 1. 1-difluorotetrachloroethane and
- 1,2-difluorotetrachloroethane;
- (C) Solidifying said melted fluorocarbon solvent and specimen at room temperature;
- and
- (D) Vacuum sublimating said solidified fluorocarbon solvent from said specimen.
4 more elements
Dependencies are inferred from the claim text.