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US 4,734,378 AGranted
Precipitation of interfering proteins in fluorescence polarization immunoassay for digoxin
AssigneeAbbott Laboratories
InventorsPhilip P. Wang, Gloria J. Hockerman
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Abstract
Interfering proteins are precipitated and digoxin extracted in a fluorescence polarization immunoassay for digoxin with a protein precipitating reagent. The reagent contains about 3 to 4% 5-sulfosalicyclic acid in an aqueous solution including about 40 to 60% of a straight or branch chained organic alcohol having from 1 to 4 carbon atoms.
Claims4
In a method for pretreating a test sample of biological fluid prior to a fluorescence polarization immunoassay for digoxin of said test sample wherein a precipitation reagent is employed to remove interfering proteins from the sample, the improvement comprising:
- intermixing said sample with a precipitation reagent comprising, in weight/volume percent, from about 3% to about 4% 5-sulfosalicylic acid in an aqueous solution having from about 40% to about 60% of a straight or branch chained organic alcohol having from 1 to 4 carbon atoms.
Dependencies are inferred from the claim text.