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US 4,734,369 AGranted
Tissue cultures of Lycopersicon spp.
AssigneeCampbell Soup Company
InventorsDavid A. Evans, William R. Sharp
Life of this patent
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Abstract
A method is provided of producing variant plant varieties of Lycopersicon spp. by tissue culture techniques employing 6-benzyladenine at high concentrations. The technique produces phenotypic variants that are the result of heritable nuclear genetic changes.
Claims15
A method of isolating heritable nuclear genetic variant plant strains comprising:
- (a) regenerating shoots from callus cells obtained from tissue of a plant on basal medium comprising 6-benzyladenine at a concentration of at least about 10 uM, said plant being from the genus Lycopersicon;
- (b) rooting said regenerated shoots on a rooting medium or directly in soil to provide plantlets;
- (c) growing said plantlets into mature plants and allowing the mature plants to self-fertilize;
- (d) collecting seed from said self-fertilized, mature plants;
- (e) growing said seed from self-fertilized plants to provide a second generation of plants;
- (f) selecting phenotypically variant plants from among said second generation of plants, said phenotypically variant plants bearing heritable nuclear genetic changes;
- and
- (g) confirming the nature of the heritable nuclear genetic changes by observing segregation ratios of phenotypically variant to wild-type siblings in said second generation, and when said segregation ratios do not conform to an approximately 3:1 or 1:3 ratio, performing backcrosses to wild-type plants and observing the progeny.
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