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US 4,734,360 AGranted
Colorimetric ethanol analysis method and test device
AssigneeLifeScan, Inc.
InventorRoger C. Phillips
Life of this patent
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Abstract
A disposable test strip device for detecting and measuring ethanol in aqueous solutions is disclosed. The test strip includes an inert support pad that contains a stabilized dry form of the enzyme alcohol oxidase, a material having peroxidative activity and an oxygen acceptor that reacts with hydrogen peroxide to give a compound of changed color. The use of this strip to determine ethanol levels colorimetrically is also disclosed.
Claims4
A method for determining the concentration of ethanol in the bloodstream of a human who is suspected of having consumed ethanol, which comprises:
- (a) reacting saliva of said human with a dry, room-temperature-stable test device comprising an inert support pad containing alcohol oxidase, mannitol present in an amount sufficient to stabilize the alcohol oxidase, a salt that buffers in the range of pH 6-9, a substance having peroxidase activity, and a substance which will react with hydrogen peroxide to give a compound of changed color, whereby a change of color is obtained in the presence of ethanol;
- and
- (b) comparing any color so obtained to a standard to determine the ethanol concentration in the bloodstream of said human.
Dependencies are inferred from the claim text.