Metal ion labeling of carrier molecules
AssigneeMallinckrodt Inc.
InventorsRobert A. Nicolotti, Alan R. Ketring, Koon Y. Pak
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Abstract
A conjugate of a protein or polypeptide and a metal ion is prepared by reacting a metal ion transfer complex comprising a chelate of 4,5-dihydroxyl-m-benzenedisulfonic acid or a salt thereof with a protein or polypeptide that is covalently bound to an exogenous chelating group having a greater affinity than 4,5-dihydroxyl-m-benzenedisulfonic acid for the metal ion.
Claims11
A method of preparing a conjugate of a protein or polypeptide and a metal ion which comprises reacting a metal ion transfer complex comprising a chelate of 4,5-dihydroxyl-m-benzenedisulfonic acid or a salt thereof and a metal ion with a protein or polypeptide that is covalently bound to an exogenous chelating group, said exogenous chelating group having a greater chelating affinity than 4,5-dihydroxyl-m-benzenedisulfonic acid for the metal ion, the reaction being carried out in an aqueous medium at a pH at which the protein or polypeptide is stable.
A method of preparing a conjugate of a protein or polypeptide and a technetium ion which comprises:
- (a) reacting 4,5-dihydroxyl-m-benzenedisulfonic acid and pertechnetate ions in an aqueous reaction medium;
- (b) electrolytically reducing the pertechnetate ions in the reaction medium of step (a) to an oxidation state less than +7;
- and
- (c) reacting the chelate of reduced technetium produced in steps (a) and (b) with a protein or polypeptide that is covalently bound to an exogenous chelating group, said exogenous chelating group having a greater chelating affinity than 4,5-dihydroxyl-m-benzenedisulfonic acid for the technetium ion, the reaction being carried out in an aqueous medium at a pH at which the protein or polypeptide is stable.
Dependencies are inferred from the claim text.