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US 4,732,862 AGranted
Determination of CEA binding substance using anti-goat immunoglobulins
OwnerAlberto Bartorelliinventor
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Abstract
A method is disclosed for the determination of human serum factors which are specific for human carcinomata antigens, in which method the serum of a patient with a carcinoma is incubated with CEA that has been radioactively labeled and anti-goat immunoglobulin serum in toto or depleted of the cross-reaction capacity for human IgA, IgG and IgM is added, then the whole mass is incubated and centrifugated, the supernatant is decanted and the precipitate is counted with a gamma counter. These specific serum factors are employed as a marker of primitive tumoral forms.
Claims5
A method for the determination of CEA binding substance (CBS), which comprises
- incubating the serum of a patient with carcinoma in a first incubation with CEA that has been radioactively labeled (.sup.125 I-CEA, 50,000 cpm, 80 .mu.Ci/.mu.g of specific activity) for at least 16 hours at room temperature;
- adding anti-goat immunoglobulin antiserum in toto or depleted of the cross-reaction capacity for human IgA, IgG and IgM;
- and
- incubating in a second incubation for 1 hour at room temperature;
- and
- centrifuging and decanting the resulting supernatant;
- and
- counting the resulting precipitate with a gamma counter.
5 more elements
Dependencies are inferred from the claim text.