Method of producing dextranase
AssigneeLouisiana State University and Agricultural and Mechanical College
InventorsDonal F. Day, David W. Koenig
Life of this patent
Estimating the term…
Abstract
A mutant species of Lipomyces starkeyi ATCC No. 12659 capable of hyperproducing dextranase and an improved method of culturing to achieve such at low pH to provide biologically contaminant free supernatant liquid containing dextranase. The further improved method of culturing the mutant species on a non-dextran, assimilable carbon source with optimal dextran induction is also disclosed.
Claims17
A culture of the strain of Lypomyces starkeyi, ATCC No. 20825, having the capability to hyperproduce extracellular dextranase about three times the dextranase production of the wild variety after 72 hours when cultured at a controlled pH in the range of from about 3.0 to about 4.0 in an aqueous nutrient medium containing assimilable carbon source, nitrogen source, and mineral source.
An improved method for producing an extracellular dextranase which comprises culturing a mutant strain of a yeast of the genus Lypomyces produced by ultraviolet light, irradiation of the parent strain Lypomyces starkeyi, ATCC No. 12659, which mutant is identified as ATCC No. 20825 and characterized by hyperproduction of dextranase of about three times the dextranase production of the wild variety after 72 hours at a controlled pH in the range of about 3.0 to 4.0 in the presence of an aqueous nutrient medium containing an assimilable carbon source.
A process for optimizing production of extracellular dextranase which process comprises culturing a strain of the yeast Lypomyces starkeyi, ATCC No. 20825 having the capability to hyperproduce dextranase at about three times the dextranase production of the wild variety after about 72 hours at a controlled pH in the range of about 3.0 to about 4.0, using a non-dextran, assimilable carbon source and adding a dextran inducer at a stage of growth identified as having an adjusted optical density of from about 8.0 to about 10.0 at 660 nm compared with water.
Dependencies are inferred from the claim text.