Immobilization of cells with a polyazetidine prepolymer
AssigneePurification Engineering, Inc.
InventorsLouis L. Wood, Gary J. Calton
Life of this patent
Estimating the term…
Abstract
A composition comprising immobilized cells obtained by applying a dispersion of cells and curable prepolymer material selected from the group consisting of polyazetidine prepolymers, carboxymethyl cellulose, polyurethane hydrogel prepolymers and polymethylene isocyanates. as a coating to a solid inert carrier and curing the prepolymer on the carrier at a temperature below the temperature at which enzyme activity of the cells is significantly reduced. The composition may be used to produce various materials such as L-aspartic acid, L-alanine, 6-Aminopenicillanic acid, high fructose corn syrup, prednisolone or phenylalanine.
Claims7
A composition comprising immobilized cells having enzyme activity, the cells being immobilized by applying an aqueous dispersion of the cells and curable polyazetidine prepolymer as a coating to a solid inert carrier and curing the prepolymer on said carrier at a temperature below the temperature at which the enzyme activity of the microbial cells is significantly reduced to immobilize said cells while retaining their enzyme activity.
A process for preparing L-alanine which comprises contacting L-aspartic acid solution with an immobilized cell/polymer composition comprising cells containing L-aspartate decarboxylase activity immobilized by means of an, insoluble, crosslinked polymer obtained by curing a curable prepolymer of polyazetidine, said composition comprising a coating on a solid inert carrier.
A process for preparing 6-Aminopenicillanic acid which comprises using an immobilized cell/polymer composition comprising cells containing penicillin G acylase activity immobilized by means of an, insoluble, crosslinked polymer obtained by curing a curable prepolymer of polyazetidine, said composition comprising a coating on a solid inert carrier.
A process for producing high fructose corn syrup which comprises using an immobilized cell/polymer composition comprising cells containing glucose isomerase activity immobilized by means of an, insoluble, crosslinked polymer obtained by curing a curable prepolymer of polyazetidine, said composition comprising a coating on a solid inert carrier.
A process for producing prednisolone which comprises using an immobilized cell/polymer composition comprising cells having steroid dehydrogenase activity immobilized by means of an, insoluble, cross-linked polymer obtained by curing a curable prepolymer of polyazetidine, said composition comprising a coating on a solid inert carrier.
A process for producing phenylalanine which comprises using an immobilized cell/polymer composition comprising cells containing phenylalanine ammonia lyase activity immobilized by means of an, insoluble, cross-linked polymer obtained by curing a curable prepolymer of polyazetidine, said composition comprising a coating on a solid inert carrier.
Dependencies are inferred from the claim text.