Monoclonal antibodies for DNA-RNA hybrid complexes and their uses
AssigneeUniversity of Hawaii
InventorsW. Dorsey Stuart, Mark B. Frank
Life of this patent
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Abstract
Monoclonal antibodies are provided capable of distinguishing DNA-RNA hybrid complexes from single stranded DNA and RNA and double stranded DNA and RNA. The antibodies find particular use in determining the presence of a specific nucleic acid sequence on a solid surface. Single stranded polynucleotide is fixed to a solid (gel) surface and then hybridized with the complementary probe. The hybrid complex specific monoclonal antibody is then added to bind to any hybrid complexes which have formed. By appropriate label, the hybrid complex may be visualized in a variety of ways.
Claims15
IgM or IgG mouse monoclonal antibodies which specifically bind to DNA-RNA hybrid complexes but which do not bind to isolated RNA or DNA.
A method for detecting the presence of a nucleotide sequence which comprises:
- fixing a DNA or RNA sequence to a solid support as a single strand;
- hybridizing said fixed single stranded nucleic acid sequence with a sequence of the other type of nucleic acid having a predetermined nucleic acid sequence to bind to any homologous fixed sequence to form a hybrid duplex;
- adding to said hybrid duplex mouse IgG or IgM monoclonal antibodies which specifically bind to DNA-RNA hybrid complexes, whereby said monoclonal antibodies bind to hybrid duplexes bound to said solid support;
- and
- determining the presence of monoclonal antibody bound to said solid support by means of a label providing a detectable signal, which label is bound directly or indirectly to said monoclonal antibody.
A kit for determining the presence in situ of a hybrid DNA-RNA complex which comprises primary IgM or IgG mouse monoclonal antibodies having the property of specifically binding to said complex but not to isolated DNA or RNA and secondary antibodies having the property specifically binding to said primary antibodies, said secondary antibodies having a label capable of providing a detectable signal.
Dependencies are inferred from the claim text.