Process for the separation of L-leucine and L-isoleucine
AssigneePolak's Frutal Works B.V.
InventorKris Bart de Roos
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Abstract
The invention relates to a process for the separation of L-leucine and L-isoleucine. The process is carried out by subjecting a mixture of leucine ester, and isoleucine ester, (or a mixture of leucine ester, isoleucine ester and valine ester) to an esterase catalyzed hydrolysis resulting in a selective hydrolysis of the leucine ester. The pure L-leucine can be isolated from the reaction mixture by first removing the non-hydrolyzed esters by extraction with an organic solvent and then crystallizing the L-leucine at its isoelectric point. The recovered L-iso-leucine ester is purified by distillation (if necessary), and hydrolyzed to yield pure crystalline L-isoleucine.
Claims19
A method of separating L-leucine from a mixture of hydrophobic amino acids, comprising:
- (a) Providing an amino acid mixture, said mixture comprising L-leucine and L-isoleucine,
- (b) esterifying said amino acid mixture with an alkanol, to form an ester mixture, said ester mixture comprising L-leucine ester and L-isoleucine ester,
- (c) selectively hydrolyzing said L-leucine ester by treating said ester mixture with an enzymatic agent selected from the group consisting of enzyme and enzyme-complex having esterase activity to form hydrolysis mixture, said hydrolyzed mixture comprising L-leucine and L-isoleucine ester and separating said L-leucine from said hydrolysis mixture to form purified L-leucine.
A method of separating L-leucine and L-isoleucine from a mixture of hydrophobic amino acids, comprising:
- (a) Providing an amino acid mixture, said mixture comprising L-leucine and L-isoleucine,
- (b) esterifying said amino acid mixture with an alcohol, to form an ester mixture, said ester mixture comprising L-leucine ester and L-isoleucine ester,
- (c) selectively hydrolyzing said L-leucine ester by treating said ester mixture with an enzymatic agent selected from the group consisting of enzyme and enzyme-complex having esterase activity to form hydrolysis mixture, said hydrolyzed mixture comprising L-leucine and L-isoleucine ester, and separating said L-leucine from said hydrolysis mixture to form purified L-leucine and an L-isoleucine ester mixture to form purified L-isoleucine.
Dependencies are inferred from the claim text.