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US 4,731,337 AGranted
Fluorimetric immunological assay with magnetic particles
AssigneeLabsystems Oy
InventorsJuhani E. I. Luotola, Hannu Harjunmaa
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Abstract
Fluorometric immunological assay method in which an antigen is attached onto magnetic particles, which are, for the time of measurement, pulled against the wall of the measurement vessel by means of the magnetic field. The magnetic particles adhering to the vessel walls are irradiated and the resultant emitted fluorescence is measured.
Claims28
A method of immunological assay for a target antibody or target antigen comprising:
- a. attaching an antigen or antibody to solid magnetic particles;
- b. contacting the magnetic particles resulting from (a) with
- (1) a sample containing either the target antibody or target antigen, and
- (2) fluorescently labeled antibody or fluorescently labeled antigen, respectively, said contacting taking place in a measurement vessel;
- c. forcing the magnetic particles resulting from (b) against the wall of the measuring vessel by an applied magnetic field;
- d. irradiating, through the walls of the measuring vessel, the magnetic particles adhering to the wall as a result of (c);
- and
- e. measuring the resultant fluorescent or phosphorescent radiation with a detector after this radiation has passed through the walls of the measuring vessel.
5 more elements
Dependencies are inferred from the claim text.