Rate method for the determination of inorganic phosphorus
AssigneeBeckman Instruments, Inc.
InventorsFrank R. Shu, James H. Francis
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Abstract
A reagent of the type comprising an acid, a molybdate salt, a non-ionic surfactant, and polyvinylpyrrolidone (PVP). The reagent is characterized in that it further comprises an enhancing amount of at least one sugar alcohol. Also, a method for assaying inorganic phosphorus of the type which comprises mixing a sample of biological fluid with a reagent and measuring the absorbance of the resulting solution. The method is characterized in that the above reagent is employed therein. The method can further be characterized in that the measurement can be made via a rate technique.
Claims14
A rate method for measuring the amount of inorganic phosphate in a sample comprising:
- (a) mixing a sample of biological fluid with a rate reagent comprising an acid, a molybdate salt, a nonionic surfactant, polyvinylpyrrolidone (PVP), and at least one sugar alcohol to produce a mixture;
- and,
- (b) measuring the rate of formation of phosphomolybdate in the mixture as a measure of the amount of inorganic phosphate in the sample.
Dependencies are inferred from the claim text.