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US 4,731,325 AGranted
Arrays of alternating nucleic acid fragments for hybridization arrays
AssigneeOrion-yhtyma Oy
InventorsAiri M. Palva, Tuula M. Ranki, Hans E. Soderlund
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AssigneeOrion-yhtyma Oy
InventorsAiri M. Palva, Tuula M. Ranki, Hans E. Soderlund
Estimating the term…
The invention is related to improved nucleic acid reagents comprising arrays of nucleic acid fragments and combinations of such fragments. The preparation of such fragments by recombinant DNA techniques and their use in sandwich hybridization methods is also described. By making different combinations of the nucleic acid fragments--some labeled and some affixed to solid carriers, it is possible to create kits for the identification of e.g. veneral diseases. The improved nucleic acid reagents comprise two series of at least two alternating nucleic acid fragments, which are homologous to sequences in the nucleic acid to be identified, one of the series being labeled and one affixed to a solid carrier. Nucleic acid fragments belonging to different series must not be homologous to each other. Sandwich hybridization tests performed with arrays of nucleic acid fragments are at least four times as sensitive as sandwich hybridization tests performed with reagents belonging to the prior art.
A nucleic acid reagent for use in a sandwich hybridization array to detect the identity of a nucleic acid, said nucleic acid reagent comprising at least two sets of at least two alternating nucleic acid fragments which are homologous to the nucleic acid which is to be identified, said alternating nucleic acid fragments being not homologous to one another, wherein at least one of the sets of nucleic acid fragments are labeled and at least one set of nucleic acid fragments are affixed to a solid carrier.
The nucleic acid reagent according to claim 21, wherein some or all of the nucleic acid fragments on one or more of the sets of nucleic acid fragments are joined to form an array of nucleic acid fragments.
Nucleic acid reagents comprising arrays of alternating nucleic acid fragments comprising the recombinant plasmid pKTH1220 or its derivatives and which recombinant plasmid contains the DNA of Chlamydia trachomatis L2 bacterium and is cloned into the host Escherichia coli K12, HB101, and the deposit number of this host comprising the recombinant plasmid pKTH1220 is DSM 2825.
Nucleic acid reagent comprising two or more series of at least two alternating arrays of nucleic acid fragments sufficiently homologous to the nucleic acid which is to be identified but not homologous to one another, comprising the recombinant plasmid pKTH1220 or its derivatives and which recombinant plasmid contains the DNA of Chlamydia trachomatis L2 bacterium and is cloned into the host Escherichia coli K12, HB101, and the deposit number of this host comprising the recombinant plasmid pKTH1220 is DSM 2825.
A method for conducting a sandwich hybridization array using a nucleic acid reagent to detect the identity of a nucleic acid, said method comprising exposing said nucleic acid to said nucleic acid reagent, said nucleic acid reagent comprising at least two sets of at least two alternating nucleic acid fragments which are homologous to the nucleic acid which is to be identified, said nucleic acid fragments being not homologous to one another, wherein the nucleic acid fragments from each of said sets alternate in said nucleic acid reagent and at least one of said sets of nucleic acid fragments are labeled and at least one set of nucleic fragments are affixed to a solid carrier.
Dependencies are inferred from the claim text.