Purifications of B-cell inducing factor (BIF) and B-cell growth factor (BGF)
AssigneeSloan-Kettering Institute for Cancer Research
InventorsPeter Ralph, Karl Welte
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Abstract
Highly purified T cell replacing factor (TRF or BIF) essentially free of IL-2 and Interferon activities is obtained in the invention. BIF is useful for treatment of immunoregulatory disorders and/or stimulation of production of immunoglobulins. A method for production of BIF and BGF is described. B cell lines for BIF assays are described as well as an assay for B cell receptor variants and a method for obtaining B cell variants from a variety of source materials.
Claims15
Method for the purification of B-cell inducing factor (BIF) which comprises:
- (a) antigen-stimulation of human immunoregulatory cells to produce BIF;
- (b) recovery of BIF from culture medium;
- (c) contacting the recovered BIF with an ion exchanger;
- (d) size-fractionation of BIF active fractions from ion-exchange;
- and
- (e) agarose chromatography of BIF active fractions derived from gel chromatography.
Purified human BIF.
Purified human BIF essentially free of IL-2 activity.
Purified human BIF essentially free of Interferon activity.
Method for the purification of B-cell growth factor (BGF) which comprises:
- (a) antigen-stimulation of human immunoregulatory cells to produce BGF;
- (b) recovery of BGF from culture medium;
- (c) contacting the recovered BGF with an ion exchanger;
- (d) size-fractionation of BGF active fractions from ion-exchange;
- and
- (e) agarose chromatography of BGF active fractions derived from gel chromatography.
Dependencies are inferred from the claim text.