Method of producing reverse transcriptase
AssigneeDirector of The National Institute of Animal Health
InventorsMichi Kodama, Kiichi Sekiguchi, Masanori Kubo
Life of this patent
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Abstract
A method of producing reverse transcriptase, which comprises isolating a fraction containing retrovirus from a tissue culture fluid supernatant of retrovirus producing cells which are able to grow and propagate in vitro, treating said fraction at least once by sucrose density gradient centrifugation to thereby obtain a purified retrovirus, and extracting reverse transcriptase from said purified retrovirus.
Claims14
A method of producing reverse transcriptase, which comprises isolating a fraction containing retrovirus from a tissue culture fluid supernatant of retrovirus producing cells, said retrovirus producing cells being Shimozuma cells IFO 50136;
- said retrovirus producing cells being grown and propagated in vitro, treating said fraction at least once by sucrose density gradient centrifugation to thereby obtain a purified retrovirus, and extracting and isolating reverse transcriptase from said purified retrovirus.
Dependencies are inferred from the claim text.