pLS010 plasmid vector
AssigneeThe United States of America as represented by the United States Department of Energy
InventorsSanford A. Lacks, Tanjore S. Balganesh
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Abstract
Disclosed is recombinant plasmid pLS101, consisting essentially of a 2.0 Kb malM gene fragment ligated to a 4.4 Kb T.sub.c r DNA fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems.
Claims3
Recombinant plasmid pLS101, deposited in the American Type Culture Collection under deposit #39938, which is characterized as a Mal.sup.+ transformant and consists essentially of a 2.0 Kb MalM gene fragment ligated to a 4.4 Kb Tc.sup.r DNA fragment and inserted in a suitable Gram-positive bacteria.
A method of producing recombinant plasmid pLS101 consisting essentially of
- digesting plasmid pLS69 into fragments;
- removing by electrophoresis a first fragment containing a 2.0 Kb and malM gene sequence;
- removing by electrophoresis a second fragment containing a 4.4 Kb Tc.sup.r DNA sequence;
- ligating said first fragment to said second fragment to form a ligation product;
- transforming a suitable Gram-positive bacteria with said ligation product.
Dependencies are inferred from the claim text.