Device for performing immunochemical assays

A device for performing immunochemical chemical assays, comprising:

• at least two transparent tubes, each having an internal surface, said tubes being disposed side by side and connected in series, thereby defining a first end and a second end for the connected tubes, at least one of the tubes having an antigen, hapten or antibody attached to the internal suface thereof;
• a syringe having a fluid passageway defined at one end thereof, and having a tubular body defining a chamber capable of receiving a fluid test sample;
• means for connecting the syringe to the first end of said connected tubes, so that the syringe chamber may be in fluid communication with said connected tubes through said fluid passageway;
• and a hollow plulnger insertable into the syringe for forcing liquid therefrom into the connected tubes, the plunger having an inner wall and an exit structure which is insertable into the fluid passageway of the syringe, the hollow plunger having first and second pistons positioned within it, a first plunger chamber being defined between the first piston and said exit structure, and a second plunger chamber being defined between the first and second pistons, the first piston having first and second spaced circumferential surfaces for engaging the inner wall of the plunger thereby defining an annular space between the first piston and the inner wall of the plunger, the inner wall of the plunger having first and second means for coacting with the first and second circumferential surfaces of the first piston when said first piston has been moved near the exit structure, said first and second means, said first piston and said inner wall of the plunger defining a fluid pathway including said annular space from the second plunger chamber to the exit structure;
• means for depressing the first and second pistons of the hollow plunger;
• and
• a waste reservoir connected to the second end of the connected tubes, the connection providing access for the tubes to fluid deposited therein so that said fluid may be back flowed through the tubes by means of suction from the syringe, the waste reservoir comprising a base member, an upright side wall and a top member;
• the base and top members having portions extending outwardly from the sidewall and having openings therein for receiving each of the at least two tubes, the base member having openings in flow communication with the reservoir for receiving the end of the syringe and for depositing fluid into the reservoir;
• conduit means in the base member connecting said openings to the first and second ends of said connected tubes, and conduit means in the base and top members for connecting the tubes in series;
• and the top member defining an opening therein for receiving the syringe.