Method for the recovery of extracellular enzymes from whole fermentation beer
AssigneeMiles Laboratories, Inc.
InventorsJack W. Brewer, Charles E. Brothers, Terry F. Farver, Chong Y. Kim, Eunkyu Lee
Life of this patent
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Abstract
Extracellular enzymes can be recovered from a whole fermentation beer by adding to the whole beer a mixture of a polymer and an inorganic salt. This total mixture produces an enzyme-rich polymer phase and a cell debris-containing, enzyme-poor salt phase which can be separated to produce an enzyme-rich product from the polymer phase.
Claims16
A process for the recovery of an extracellular enzyme from whole fermentation beer which comprises adding to whole fermentation beer containing microorganism cells and an extracellular enzyme a mixture of (a) a polymer selected from the class consisting of polyethylene glycol, an amine derivative of polyethylene glycol, a carboxylate derivative of polyethylene glycol, polypropylene glycol, an amine derivative of polypropylene glycol, a carboxylate derivative of polypropylene glycol, poly (ethylene glycol) ester, polyethyleneimine, trimethylamino-polyethylene glycol, polyvinyl alcohol, polyvinylpyrrolidone and mixtures thereof, and (b) an inorganic salt, allowing the whole fermentation beer-polymer-salt mixture to separate into an enzyme-rich polymer phase and an enzyme-poor salt phase, and recovering an enzyme-rich product therefrom.
A process for the recovery of an extracellular alkaline protease which comprises fermenting a suitable strain of Bacillus licheniformis in an appropriate nutrient medium to produce a whole fermentation beer containing Bacillus licheniformis cells and extracellular alkaline protease, adding to said whole fermentation beer 2.5 percent calcium chloride dihydrate and 0.6 percent monosodium or monopotassium phosphate, said percentages being on a weight/volume basis calculated on the weight of additive to volume of fermentation beer, adding to the above mixture 3 percent polyethylene glycol and 15 percent sodium sulfate, said percentages being by weight based on the total mixture weight of fermentation beer and additives, forming an alkaline protease-rich polyethylene glycol phase and an alkaline protease-poor sodium sulfate phase, and separating the two phases to recover an alkaline protease-rich product therefrom.
A process for the recovery of an extracellular heat-stable alpha-amylase which comprises fermenting a suitable strain of Bacillus licheniformis in an appropriate nutrient medium to produce a whole fermentation beer containing Bacillus licheniformis cells and extracellular alpha-amylase, adding to said whole fermentation beer 0.4 percent calcium hydroxide, 0.12 percent monosodium or monopotassium phosphate and 4 percent calcium chloride dihydrate, said percentages being on a weight/volume basis calculated on the weight of additive to volume of fermentation beer, adding to the above mixture 3.5 percent polyethylene glycol, 10 percent sodium chloride and 8 percent sodium sulfate, said percentages being by weight based on the total mixture weight of fermentation beer and additives, forming an amylase-rich polyethylene glycol phase and an amylase-poor sodium chloride-sodium sulfate phase and separating the two phases to recover an amylase-rich product therefrom.
A process for the recovery of an extracellular alpha-amylase which comprises fermenting a suitable strain of Bacillus amyloliquefaciens in an appropriate nutrient medium to produce a whole fermentation beer containing Bacillus amyloliquefaciens cells and extracellular alpha-amylase, adding to said whole fermentation beer 3 percent calcium chloride dihydrate, 0.3 percent calcium hydroxide and 0.6 percent monosodium or monopotassium phosphate, said percentages being on a weight/volume basis calculated on the weight of additive to volume of fermentation beer, adding to the above mixture 3.15 percent polyethylene glycol, 10 percent sodium chloride and 13.5 percent sodium sulfate, said percentages being by weight based on the total mixture weight of fermentation beer and additives, forming an amylase-rich polyethylene glycol phase and an amylase-poor sodium chloride-sodium sulfate phase and separating the two phases to produce an amylase-rich product therefrom.
Dependencies are inferred from the claim text.