Method for producing L-glutamic acid
AssigneeTakeda Chemical Industries, Ltd.
InventorsYukihiro Kanegae, Yoshio Sugiyama, Isamu Nakatsui
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Abstract
L-Glutamic acid is produced in a high yield by cultivating an L-glutamic acid-producing microorganism which requires oleic acid but does not require biotin for growth in a culture medium containing an oleic acid compound and a biotin compound of no less than 100 .mu.g/liter as biotin, with carbohydrate and acetic acid as carbon sources being maintained in a weight ratio of about 80:20 through about 40:60.
Claims7
A method for producing L-glutamic acid, which comprises cultivating an L-glutamic acid-producing microorganism which requires oleic acid but does not require biotin for growth in a culture medium containing an oleic acid compound and a biotin compound of no less than 100 .mu.g/liter as biotin, with carbohydrate and acetic acid as carbon sources being maintained in a weight ratio of about 70:30 through about 50:50, said microorganisms being from the group consisting of Brevibacterium thiogenitalis D248, Ferm BP-433, Brevibacterium flavum BN-11, Ferm BP-435, and Corynebacterium glutamicum No. 534-MS-023, Ferm BP-434.
Dependencies are inferred from the claim text.