Recombinant growth hormone releasing factor
AssigneeHoffmann-La Roche Inc.
InventorsRam S. Bhatt, Kenneth J. Collier, Robert M. Crowl, Mohindar S. Poonian
Life of this patent
Estimating the term…
Abstract
Th preparation of a double-stranded polydeoxy ribonucleotide having cohesive termini each comprising one strand of a double-strand restriction endonuclease recognition site and, between the termini, a structural gene coating for the expression of recombinant growth hormone releasing factor, at least a majority of the codons and the coding strand of said gene being codons preferred for the expression of microbial genomes; a method of producing such DNA a recombinant microbial cloning vehicle comprising such DNA and the novel methods of producing said peptide are described.
Claims27
A recombinant double-stranded DNA molecule having cohesive termini with each terminus comprising a single strand of a double stranded restriction endonuclease recognition site and between the termini, a structural gene coding for human GRF-OH (1-44) or a biologically active fragment of human GRF of at least 37 amino acids that stimulates secretion of human growth hormone releasing factor.
A recombinant DNA sequence coding for human GRF-OH (1-44) or a biologically active fragment of human GRF of at least 37 amino acids that stimulates secretion of human growth hormone releasing factor operably linked with a DNA sequence capable of effecting microbial expression of said human GRF or said biologically active fragment.
A recombinant DNA microbial expression vehicle comprising promoter and operator DNA sequences and a DNA sequence coding for human GRF-OH (1-44) or a biologically active fragment of human GRF of at least 37 amino acids that stimulates the secretion of human growth hormone releasing factor, said replicable expression vehicle capable, in a transformant microorganism, of expressing said human GRF or said biologically active fragment.
The structural gene coding for GRF-OH(44) consisting of the nucleotide sequence set forth in FIG. 2.
A structural gene coding for GRF-OH(40) consisting of the nucleotide sequence obtained by ligating the gene segment set forth in FIG. 14 formed from fragment 14", 15" and 16" with gene fragments 1-13 of FIG. 4.
A structural gene coding for GRF-OH(37) consisting of the nucleotide sequence obtained by ligating the gene segment set forth in FIG. 13 formed from fragments 14', 15' and 16' with gene fragments 1-13 of FIG. 4.
Dependencies are inferred from the claim text.