Process and reagent for the determination of transaminase
AssigneeBoehringer Mannheim GmbH
InventorGerald Moller
Life of this patent
Estimating the term…
Abstract
For the determination of the transaminasae by reaction of a corresponding amino acid with .alpha.-ketoglutaric acid to form glutamate and the .alpha.-keto acid corresponding to the amino acid used, and measurement of the .alpha.-ketonic acid formed by reduction in the presence of lactate dehydrogenase (LDH) and optionally of malate dehydrogenase (MDH) with NADH, forming NAD.sup.+ and .alpha.-oxy acid, use is made of D-LDH from lactic acid bacterium.
Claims13
A process for the determination of transaminase in a sample comprising contacting said transaminase containing sample with alpha-ketoglutaric acid and at least one amino acid to form glutamate and a transaminated alpha-ketoacid, further contacting said sample with D-lactate dehydrogenase obtained from lactic acid producing bacteria and NADH, said NADH reducing said transaminated alpha-keto acid product to an alpha oxyacid and being oxidized to NAD.sup.+, and determining the type of alpha oxyacid produced or measuring the rate of formation of NAD.sup.+ or alpha oxyacid to determine the type of transaminase or amount of transaminase present.
Composition useful in determining the presence of a transaminase comprising D-lactate dehydrogenase obtained from a lactic acid producing bacteria, alpha-oxoglutarate, an amino acid selected from the group consisting of alanine and aspartate, NADH and a buffer at a pH from 6.5 to 8.5.
Dependencies are inferred from the claim text.