Sparger for fermentation and tissue culturing vessels
AssigneeNew Brunswick Scientific Co., Inc.
InventorsDavid Freedman, Zheng Zhenbin, Shaul Reuveny
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Abstract
A sparger for use in microcarrier fermentation systems is provided which includes a narrow, vertically extending outer chamber defined by a solid wall and a wall formed by a screen, wherein air or other gas is forced under pressure through a tube to the bottom of the chamber and from there to a distribution member for the release of the gas upwardly into the chamber. The top of the chamber is provided with exit openings which communicate to a point above the surface of the medium. In an alternate embodiment, gas is released at the bottom of an inner chamber defined by a wall formed with a plurality of screened windows.
Claims25
A sparger for cell culture reactor systems containing a liquid comprising a vertically extending chamber positioned in a liquid, said chamber defined by at least a first wall formed at least in part by a screen, and means for directing a gas into the bottom of the chamber in order to aerate said liquid, said aerated liquid passing through said screen and exit opening means proximate the top of the chamber for communication with a position above the liquid level in the cell culture reactor system.
Dependencies are inferred from the claim text.