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US 4,727,035 AGranted
Immunoassay for cyclosporin
OwnerWalter C. Mahoneyinventor
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Abstract
An immunoassay is disclosed which measures the concentration of cyclosporin analytes in sample fluids. Also disclosed is radioiodinated cyclosporin having a specific activity greater than 50 ulCi/ug and fluorescent labeled cyclosporin, both of which is cross-reactive with cyclosporin analytes.
Claims12
A method of immunoassay useful for measuring the concentration of cyclosporin analytes in sample fluids comprising:
- competitively reacting labeled cyclosporin and a cyclosporin analyte with an antibody specific to the cyclosporin analyte and the labeled cyclosporin, the quantity and specific activity of the labeled cyclosporin and the quantity and reactivity of the antibody being sufficient to give a measurable detection signal representative of the ratio of antibody bound labeled cyclosporin to unbound labeled cyclosporin
- wherein the labeled cyclosporin is labeled cyclosporin A, labeled cyclosporin B, labeled cyclosporin C or labeled cyclosporin D and the labeled cyclosporin is labeled with a radioiodine label or a fluorescent label.
As an article of manufacture, an immunoassay kit useful for measuring the concentration of cyclosporin analytes in sample fluids comprising:
- at least one container with labeled cyclosporin, wherein the labeled cyclosporin is labeled cyclosporin A, labeled cyclosporin B, labeled cyclosporin C or labeled cyclosporin D and the labeled cyclosporin is labeled with a radioiodine label or a fluorescent label;
- and
- at least one container with an antibody specific to the labeled cyclosporin.
Dependencies are inferred from the claim text.