Process and reagent for the fully enzymatic determination of urea
AssigneeBoehringer Mannheim GmbH
InventorsJoachim Siedel, August W. Wahlefeld, Joachim Ziegenhorn
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Abstract
For the enzymatic determination of urea, one reacts the urea with glyoxylate in the presence of ureidoglycolate synthetase to give (S)-ureidoglycolate and oxidizes the latter with NAD(P).sup.+ and ureidoglycolate dehydrogenase to carbamoyloxamate and measures NAD(P)H formed either directly or via a color indicator system. A reagent suitable herefor contains glyoxylate, NAD(P).sup.+, (S)-ureidoglycolate synthetase, ureidoglycolate dehydrogenase and buffer substance.
Claims12
Process for the enzymatic determination of urea, comprising reacting a urea containing sample with glyoxylate in the presence of (S)-ureidoglycolate synthetase to form (S)-ureidoglycolate, oxidizing the (S)-ureidoglycolate with NAD(P).sup.+ and ureidoglycolate dehydrogenase to form carbamoyloxamate and NAD(P)H and measuring NAD(P)H formed as a measure of urea in said sample.
A reagent for the enzymatic determination of urea, comprising glyoxylate, NAD(P).sup.+, (S)-ureidoglycolate synthetase, ureidoglycolate dehydrogenase and a buffer substance.
Dependencies are inferred from the claim text.