Preparations for use in solid phase immunoassays comprising monoclonal antibodies covalently embedded in their immobilized hybridoma cells

A process for the preparation of a solid reagent for use in diagnostic immunoassay, comprising incubating in vitro a culture medium containing active hybridoma cells capable of producing monoclonal antibodies, allowing the formation of antibodies to proceed, separating and washing said cells free of serum, resuspending the cells in a buffer solution, adding a fixative to the resulting suspension, thus covelently binding to said hybridoma cells these self-produced monoclonal antibodies which were incapable of removal from said hybridoma cells by said washing and resuspending steps theretby to produce an immobilized fixed hybridoma cells/monoclonal antibodies complex, binding excessive free inactivator substance and isolating said immobilized fixed hybridoma cells/monoclonal antibodies complex.

A reagent for use in solid phase immunoassay diagnostics consisting essentially of a matrix of fixed hybridoma cells embedded with and covalently bound to its self-produced monoclonal antibodies, said bound monoclonal antibodies presenting sites available for binding to a specific antigen.