Plasma fraction purification using silica resin bound to a ligand
AssigneeAlpha Therapeutic Corporation
InventorSteven W. Herring
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Abstract
There is provided, in accordance with practice of this invention, a process for separating Factor IX and/or Factor X from an impure protein fraction containing protein in addition to Factors IX and X. A silica resin coupled with a ligand capable of binding Factor IX and/or Factor X is provided. An aqueous solution of the impure protein fraction is applied to the ligand-coupled silica resin to thereby bind the Factor IX and/or Factor X to the resin. The Factor IX and/or Factor X is then recovered from the resin by elution.
Claims28
A process for separating Factor IX from an impure protein fraction containing Factor IX, the process including the steps of:
- providing an aqueous solution of the impure protein fraction;
- applying the impure protein fraction solution to a chromatographic column containing a silica resin coupled with a ligand capable of binding Factor IX;
- binding Factor IX to the ligand;
- and
- recovering Factor IX from the column.
A process for separating of Factor X from an impure protein faction containing Factor X, the process including the steps of:
- providing an aqueous solution of the impure protein fraction;
- applying the impure protein fraction solution to a chromatographic column containing a silica resin coupled with a ligand capable of binding Factor X;
- binding Factor X to the ligand;
- and
- recovering Factor X from the column.
A process for separating Factor IX from an impure protein fraction containing Factor IX, the process including the steps of:
- preparing an aqueous solution of the impure protein fraction;
- preparing a silica resin coupled with a ligand capable of binding Factor IX;
- applying the impure protein fraction solution to the ligand-coupled silica resin;
- binding Factor IX to the ligand;
- and
- recovering Factor IX from the resin.
A process for separating of Factor X from an impure protein faction containing Factor X, the process including the steps of:
- preparing an aqueous solution of the impure protein fraction;
- preparing a silica resin coupled with a ligand capable of binding Factor X;
- applying the impure protein fraction solution to the ligand coupled silica resin;
- binding Factor X to the ligand;
- and
- recovering Factor X from the resin.
Dependencies are inferred from the claim text.