# US 4,725,548 A · Method and fermenter for growing tissue cells

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- Number: US 4,725,548 A
- Document ID: US4725548A
- Status: Granted
- Kind: A
- Application number: 06/860,689
- Filed: 1986-05-02
- Granted: 1988-02-16
- Earliest claimed: 1983-05-16
- Assignees: Chemap AG
- Inventors: Daniel Karrer
- Web page: https://patentgrep.com/patent/US4725548A

## CPC

- C12M 29/04: {Filters; Permeable or porous membranes or plates, e.g. dialysis}
- C12M 33/14: {with filters, sieves or membranes}
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}

## Abstract

In submerged growing of tissue cells on microcarriers in a culture medium, the culture medium is alternately aerated or withdrawn from a bottom of a culture container via a sintered plate. An air chamber is formed under the sintered plate.

## Claims

### Claim 1

A method of submerged growing of tissue cells on microcarriers in a culture medium, comprising the steps of
providing a culture container having a bottom;
filling the culture container with a culture medium and a microcarrier;
providing in the bottom of the container a sintered plate formed so that it allows for aerating the culture medium in the culture container through the sintered plate with simultaneous cleaning of the sintered plate and also allows withdrawing of the culture medium from the container through the sintered plate;
providing culture medium aerating and sintered plate cleaning means, and culture medium withdrawing means outside of the culture container; and
alternately connecting the culture aerating and sintered plate cleaning means, and the culture medium withdrawing means with the sintered plate so as to provide alternately aerating the culture medium in the culture container through said sintered plate and clean the sintered plate, and withdrawing the culture medium from the container through the sintered plate.