Back to results
US 4,725,544 AGranted
Method for purifying xylanase
OwnerLarry U. Tan, John N. Saddler +1inventors
InventorsLarry U. Tan, John N. Saddler, Ernest K. Yu
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
OwnerLarry U. Tan, John N. Saddler +1inventors
InventorsLarry U. Tan, John N. Saddler, Ernest K. Yu
Estimating the term…
The separation of xylanases from mixtures thereof with other hemicellulases, particulary cellulase produced by the culturing of hemicellulolytic microorganisms, particularly the fungus Trichoderma harzianum E58 and Trichoderma reesei by ultrafiltration through an ultrafiltration membrane having a low molecular weight cut-off point in the range of about 1,000 to 20,000 daltons to obtain a cellulase rich retentate and xylanase rich ultrafiltrate. The dilute xylanase rich filtrate from the ultrafiltration is concentrated and purified by adsorption and elution from an insoluble matrix, e.g. a cationic exchange resin. The xylanase obtained is suitable for use in the hydrolysis of hemicellulose for which it is selective, particularly in the presence of cellulose and the cellulase rich retentate is suitable for the hydrolysis of cellulose.
A process for the separation of xylanase from a mixture thereof with cellulase, said mixture being produced by the culturing of a hemicellulolytic microorganism which comprises subjecting said mixture to ultrafiltration through an ultrafiltration membrane having a low molecular weight cutoff point between 1,000 and 20,000 daltons to obtain a xylanase rich ultrafiltrate and a cellulase rich retentate, and concentrating and purifying the ultrafiltrate by adsorption and elution from an insoluble matrix to produce a highly purified concentrated xylanase.
Dependencies are inferred from the claim text.