Back to results
US 4,722,840 AGranted
Hybrid particle immunogens
AssigneeChiron Corporation
InventorsPablo D. T. Valenzuela, George Kuo, Philip J. Barr
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeChiron Corporation
InventorsPablo D. T. Valenzuela, George Kuo, Philip J. Barr
Estimating the term…
Novel immunogenic compositions are provided involving viral particles composed at least in part of hybrid proteins of at least a portion of a particle forming protein and one or more polypeptides having at least one epitope of interest. Nucleic acid sequences are employed coding for the hybrid protein which are introduced into a host cell for expression, either by themselves or in combination with other DNA sequences coding for particle forming proteins. Expression of the DNA sequences results in formation of particles which may be isolated and used as immunogens for production of antibodies for diagnostics purposes, passive immunization, vaccination, or other uses. Saccharomyces carlsbergensis, 2150-2-3 (pDC103), was deposited on Sept. 7, 1984, at the ATCC and given ATCC Accession No. 20726. Also, Saccharomyces cerevisiae PO17 (pC1/1-MCS29) was deposited at the ATCC on Sept. 5, 1985, and given ATCC Accession No. 20770.
A hybrid polypeptide comprising an assembled particle, said hybrid polypeptide comprised of a particle-forming fragment of a structural protein from a virus particle fused to a heterologous amino acid sequence defining an eptopic site of a pathogen or toxin, said heterologous amino acid sequence being disposed within said assembled particle such that said eptopic site is immunologically reactive.
A novel hybrid polypeptide comprising at least a major portion of the Hepatitis B surface antigen fused to one or more oligopeptides, where the hybrid polypeptide is capable of forming a particle in a cellular host upon expression with presentation of at least one epitope of at lest one oligopeptide.
A novel hybrid polypeptide comprising Hepatitis B surface antigen linked to an eptopic region of the envelope D protein of Herpes Simplex virus or a circumsporozite protein of Plasmodium falciparum through at least a portion of the pre-surface polypeptide.
Dependencies are inferred from the claim text.