Purfication of blood clotting factors and other blood proteins on non-carbohydrate sulfated matrices
AssigneeMiles Laboratories, Inc.
InventorRobert E. Jordan
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Abstract
There is disclosed a non-carbohydrate sulfated matrix and the use thereof to isolate and purify blood clotting factors and other blood proteins by a procedure involving the adsorption of at least one of such factors and proteins onto the non-carbohydrate sulfated matrix followed by elution of such factors and proteins onto the non-carbohydrate sulfated matrix followed by elution of such factors and proteins from the matrix.
Claims7
In a method of isolating and purifying at least one of the group of blood coagulation factors and proteins selected from the group consisting of factors IX, X, II, VII, VIII, protein C, protein S, prothrombin, and tissue plasminogen activator, from a source of said blood coagulation factors and proteins selected from blood, blood plasma, plasma concentrates, and tissue culture fluids, obtained by means of biotechnology techniques, by the steps of
- (a) contacting the blood, blood plasma, plasma concentrates, and tissue culture fluids with at least one affinity chromatography adsorbing agent,
- (b) separating the non-adsorbed solution from the non-adsorbing agent, and
- (c) eluting the adsorbed blood coagulation factors and proteins from the adsorbing agent using an eluant effective to elute the desired factor(s) and protein(s),
- the improvement comprising using as the adsorbing agent a sulfated, synthetic, non-carbohydrate affinity chromatography gel matrix adsorbing agent comprising a sulfatable polymer, said sulfatable polymer selected from the group consisting of
- (i) a synthetic organic affinity chromatography gel matrix prepared by polymerization of at least one polymerizable organic monomer, and
- (ii) polymeric silica particles,
- said sulfatable polymer having been treated by a process consisting essentially of treating with a sulfating agent so as to provide a multiplicity of sulfate groups bound directly to the polymer backbone.
A sulfated, synthetic, noncarbohydrate affinity chromatography gel matrix adsorbing agent comprising a sulfatable polymer, said sulfatable polymer selected from the group consisting of a synthetic organic affinity chromatography gel matrix prepared by polymerization of at least one polymerizable organic monomer, and polymeric silica particles, said sulfatable polymer having been treated by a process consisting essentially of treating with a sulfating agent so as to provide a multiplicity of sulfate groups bound directly to the polymer backbone.
Dependencies are inferred from the claim text.