Genetic probe used in the detection of adrenal hyperplasia
OwnerPerrin C. White, Bo Dupont +1inventors
InventorsPerrin C. White, Bo Dupont, Maria I. New
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Abstract
A cDNA clone is isolated encoding a bovine adrenal cytochrome P-450 specific for steroid 21-hydroxylation (P-450.sub.C21). This plasmid pC21a contains an insert of 520 base pairs. It hybridizes with mRNA encoding P-450.sub.C21. The peptide encoded by the insert is highly homologous to two peptides isolated from porcine P-450.sub.C21 and shows limited homology to the P-450 induced by phenobarbital in rat liver. This probe and its human equivalents can be used to screen in utero i.e. pre-natal as well as post-natal patients for adrenal hyperplasia since it reacts well with human DNA as shown. These probes can also be useful for an industrial fermentation process to produce cortisone in quantity.
Claims3
cDNA probe pC21a (ATCC 39663) capable of binding to genomic human DNA diagnostic for congenital C21 hydroxylase deficiency adrenal hyperplasia in humans.
Method for detection of congenital adrenal hyperplasia C-21 hydroxylation defect in humans which comprises:
- contacting samples of human genomic DNA with a labelled pC21a cDNA probe (ATCC 39663) specific for genomic DNA coding for cytochrome P-450 steroid 21 hydroxylase for a time and under conditions sufficient to effect hybridization and observing the presence or absence of labelled probe binding to the genomic DNA, the absence of binding indicating a congenital adrenal hyperplasia C-21 hydroxylation defect.
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