Plastic roller bottle for suspension cultures

A plastic bottle for suspension cultures, which comprises:

• a closed body defining a longitudinal axis and having flexible plastic walls;
• aseptically sealable means for access into said closed body;
• and a plurality of spaced reinforcement rings defined in said flexible plastic walls about said longitudinal axis, to cause said body to normally retain a predetermined, generally cylindrical shape, the flexible plastic walls being made of a plastic formulation and being generally of a thickness, at least apart from said rings, such that an oxygen diffusion of at least 40 cc. and a carbon dioxide diffusion of at least 150 cc. is provided per square meter per hour through said plastic walls.

A plastic bottle for suspension cultures, which comprises:

• a closed body defining a longitudinal axis and having flexible plastic walls;
• aseptically sealable means for access into said closed body;
• and a plurality of spaced reinforcement rings defined in said flexible plastic walls about said longitudinal axis, to cause said body to normally retain a predetermined, generally cylindrical shape, the flexible plastic walls being made of a plastic formulation and being generally of a thickness such that an oxygen diffusion of at least 40cc. and a carbon dioxide diffusion of at least 150cc. is provided per square meter per hour of said plastic walls, at least apart from said rings, there being from 400 to 1000 square centimeters of said plastic walls apart from said rings present, said aseptically sealable access means comprising at least one flexible, thermoplastic access tube communicating through said closed body at one end thereof, said access tube having a free end which is sealed to sterile connector means.