US 4,714,763 A · Novel oxazine-ureas and thiazine urea chromophors as fluorescent labels

Abstract

Novel urea derivatives of oxazine and thiazine chromophors have the structural formula I or II. ##STR1## wherein M is oxygen or sulfur, R.sub.1 and R.sub.2 are aliphatic alkyl groups or hydrogen; R.sub.3 is hydrogen or alkyl group; R.sub.4 is hydrogen, alkyl or amine group; R.sub.5 is hydrogen, amine or alkyl group; X.sup..crclbar. is an anion consisting of an organic (e.g. CH.sub.3 COO.sup..crclbar., CH.sub.3 CH.sub.2 COO.sup..crclbar. and the like) or inorganic specie (e.g. Cl.sup..crclbar., Br.sup..crclbar., I.sup..crclbar., ClO.sub.4.sup..crclbar., SO.sub.4 ", and the like); n is 0 to 20; Z is N.dbd.C.dbd.O, N.dbd.C.dbd.S, carboxylic, primary or secondary amine, and when n=0, Z may be ##STR2## wherein Q is hydroxyl, amino, carboxylic, sulfydryl, isocyanato, or isothiocyanato. The functional oxazine-urea and thiazine-urea derivatives react with compounds of interest to form adducts resulting in the fluorescent labeling of the compound.

Claims8

Dependent claims

Urea derivatives of oxazine and thiazine chromophors selected from the group consisting of:

##STR21## wherein n is 0 to 20;
M is oxygen or sulfur;
R.sub.1 is hydrogen or alkyl group from 1 to 10 carbon atoms;
R.sub.2 is hydrogen or alkyl group from 1 to 10 carbon atoms;
R.sub.3 is hydrogen or alkyl group from 1 to 10 carbon atoms;
R.sub.4 is hydrogen, alkyl group from 1 to 10 carbon atoms, halogen or amino group;
R.sub.5 is hydrogen, alkyl group from 1 to 10 carbon atoms, halogen or amino group;
X.sup..crclbar. is an organic or an inorganic anion and Z is N.dbd.C.dbd.O, N.dbd.C.dbd.S, carboxylic, or amino, and when n=0, Z may be ##STR22## wherein Q is isocyanato or isothiocyanato.

5 more elements

Dependencies are inferred from the claim text.

Novel oxazine-ureas and thiazine urea chromophors as fluorescent labels

Life of this patent

Novel oxazine-ureas and thiazine urea chromophors as fluorescent labels

Life of this patent

Abstract

Claims8