Process for purification of phospholipids
AssigneeThe Liposome Company, Inc.
InventorsPaul A. Tremblay, John J. Kearns
Life of this patent
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Abstract
A process for the separation and purification of individual phospholipids, especially phosphatidylcholine or lecithin and phosphatidylethanolamine, from mixtures containing members of the sub-class of phosphatides, incorporating methods of solvent extraction appropriate to the scale of the sample and utilizing an acetonitrile, acetonitrile-hydrocarbon, or acetonitrile-fluorocarbon solvent, which exhibit differential solubility properties towards the individual phospholipids.
Claims44
A process for purifying individual phospholipids from mixtures thereof, which comprises separating phosphatidylcholines from the phospholipid mixtures by non-chromatographic extraction with acetonitrile.
A process for purifying phospholipids, comprising:
- (a) extracting phosphatidylcholines from egg yolks with acetonitrile;
- (b) evaporating the acetonitrile to form a residue;
- and
- (c) preciptating phosphatidylcholines from the residue with acetone.
A process for purifying phospholipids, comprising:
- (a) extracting phosphatidylcholines from egg yolks with acetonitrile;
- (b) evaporating from about 75 to about 95% of the acetonitrile to produce an oiled-out fraction, which fraction is removed from the remaining acetonitrile;
- (c) dissolving the oiled-out fraction in hexane;
- and
- (d) precipitating phosphatidylcholines from the hexane solution with acetone.
A process for purifying phospholipids, comprising:
- (a) washing egg yolks with acetone to form a residue;
- (b) extracting phosphatidylcholines from the residue with acetonitrile;
- (c) evaporating the acetonitrile to form a second residue;
- and
- (d) precipitating phosphatidylcholines from the second residue of step (c) with acetone.
A process for purifying phospholipids, comprising:
- (a) washing egg yolks with acetone to form a residue;
- (b) extracting phospholipids from the residue with an organic solvent pair selected from the group consisting of chloroform-methanol, methylene chloride-methanol, methylene chloride-ethanol, hexane-methanol, hexane-ethanol, trichlorofluromethane-ethanol and ethanol-diethyl ether;
- (c) adding water to the extract of step (b) to form a biphasic system;
- (d) collecting the organic phase of the biphasic system and evaporating the organic solvent to form a second residue;
- (e) precipitating the phosphatides from the second residue of step (d) with acetone;
- and
- (f) extracting phosphatidylcholines from the residue of step (e) using acetonitrile.
A process for puriying phospholipid, comprising:
- (a) washing egg yolks with acetone to form a residue;
- (b) extracting phopspholipids from the residue with an organic solvent pair selected from the group consisting of chloroform-methanol, methylene chloride-methanol, methylene chloride-ethanol, hexane-methanol, hexane-ethanol, trichlorofluromethane-ethanol and ethanol-diethyl ether;
- (c) adding water to the extract of step (b) to form a biphasic system;
- (d) collecting the organic phase of the biphasic system and evaporating the organic solvent to form a second residue;
- (e) extracting the second residue of step (d) with acetonitrile;
- and
- (f) precipitating phosphatidylcholines from the extract of step (e) with acetone.
A process for purifying individual phospholipids from mixtures thereof, comprising extracting the mixtures of phospholipids with acetonitrile combined with a hydrocarbon solvent.
A process for purifying individual phospholipids from mixtures thereof, comprising extracting the mixtures of phospholipids with acetonitrile combined with a flurocarbon solvent.
Dependencies are inferred from the claim text.