Particle separation process
AssigneeE. I. Du Pont de Nemours and Company
InventorGeorge F. Dunn, Jr.
Life of this patent
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Abstract
A process for separating particles from mixtures containing the particles is disclosed. An adoptive transfer therapy process wherein mononuclear cells are separated from a mixture of blood cells by the process is also disclosed. The process comprises supporting the mixture in a centrifuge chamber; subjecting the chamber to a centrifugal force; and forcing a displacing fluid through the chamber, against the centrifugal force, thereby separating the preselected particles from the mixture. The process is further characterized in that the displacing fluid has a density greater than or equal to the density of the preselected particles.
Claims32
A process for separating preselected particles from a mixture containing the particles, comprising:
- supporting the mixture in a centrifuge chamber;
- subjecting the chamber to a centrifugal force;
- and
- forcing a displacing fluid through the chamber, against the centrifugal force, thereby separating the preselected particles from the mixture;
- wherein the displacing fluid has a density greater than or equal to the density of the preselected particles.
In an adoptive transfer therapy process wherein mononuclear cells are contacted with a lymphokine to activate lymphokine-activated killer cells in the mononuclear cells, the improvement comprising separating said mononuclear cells from a mixture of blood cells by the process comprising:
- supporting the mixture of blood cells in a centrifuge chamber;
- subjecting the chamber to a centrifugal force;
- and
- forcing a displacing fluid through the chamber, against the centrifugal force, thereby separating mononuclear cells from the mixture;
- wherein the displacing fluid has a density greater than or equal to the density of the mononuclear cells.
Dependencies are inferred from the claim text.