Composition and method for rapid detection of microorganisms in clinical samples
AssigneeBecton, Dickinson and Company
InventorsJames D. Mansour, Thomas H. Schulte, Vernon R. Neece
Life of this patent
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Abstract
A method for rapid detection of microorganisms in a body fluid sample includes detecting the microorganisms after treatment of the sample with a lysing agent in order to dissolve sample components other than microorganisms, and staining with a fluorescent dye. The lysing agent may be part of a composition which includes a culture medium thereby providing simultaneous lysis and growth before staining. Detection is preferably accomplished by reliance on the fluorescence emitted by the dye having been properly excited by light energy. A composition suitable for use in the above-described method includes a growth medium and a lysing agent.
Claims18
A method for the detection of microorganisms in a body fluid sample comprising mixing said body fluid sample with an enzyme in a buffer whereby components of said body fluid but substantially none of the microorganisms in said sample are lysed, staining said microorganisms with ethidium bromide and determining the presence of said microorganisms by detecting the fluorescence emitted by said ethidium bromide.
A method for the detection of microorganisms in a body fluid sample comprising mixing said body fluid sample with an enzyme in a buffer whereby components of said body fluid but substantially none of the microorganisms in said sample are lysed, staining said microorganisms with a fluorescent dye, and determining the presence of said microorganisms by detecting the fluorescence emitted by said fluorescent dye.
A method for the detection of microorganisms in a body fluid sample comprising the steps of:
- (a) mixing said body fluid sample with an enzyme in a buffer to provide a mixture wherein components of said body fluid but substantially none of the microorganisms are lysed;
- (b) incubating said mixture;
- (c) contacting said mixture with a staining composition comprising ethidium bromide and a staining buffer;
- (d) directing excitation light to said mixture;
- and
- (e) detecting the fluorescence emitted by said ethidium bromide.
A method for the detection of microorganisms in a body fluid sample comprising the steps of:
- (a) mixing the body fluid sample with 5XLA lysing agent to provide a mixture wherein components of said body fluid but substantially none of the microorganisms are lysed;
- (b) incubating said mixture;
- (c) adding a staining buffer and ethidium bromide to said mixture;
- and
- (d) flowing said mixture through a fluorescence activated flow cytometer and detecting the fluorescence associated with the microorganisms in said mixture.
Dependencies are inferred from the claim text.