Magnetic carbohydrate particles as carriers for affinity separation purposes
AssigneeGambro Lundia AB
InventorUlf Schroder
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Abstract
Magnetically responsive spheres having an average diameter less than 1,000 nm are prepared by dissolving a carbohydrate polymer in a polar solvent, suspending magnetic material in the dissolved carbohydrate, emulsifying the suspension with an emulsion liquid to form an emulsion containing droplets of the suspension and then contacting the emulsion with a crystallizing liquid capable of crystallizing the carbohydrate polymer. The resulting spheres may be bonded with a bioabsorptive material and can be employed in processes such as cell separation, affinity purification or immunochemical assays.
Claims10
A method for preparing magnetically responsive spheres comprising the steps of dissolving a carbohydrate polymer in a polar solvent therefor so as to obtain a dissolved carbohydrate;
- suspending magnetic material in said dissolved carbohydrate so as to obtain a suspension of magnetic material in said dissolved carbohydrate;
- emulsifying said suspension with an emulsion liquid which is immiscible with said suspension so as to form an emulsion containing droplets of said suspension in said emulsion liquid;
- stabilizing said emulsion by contacting said emulsion with a crystallizing liquid different than said polar solvent to thereby crystallize said carbohydrate polymer so as to enclose said magnetic material in spheres of said carbohydrate polymer having an average diameter of less than 1,000 nm;
- and separating said spheres therefrom.
A method for preparing magnetically responsive spheres comprising the steps of dissolving a carbohydrate polymer in a polar solvent therefor so as to obtain a dissolved carbohydrate;
- suspending magnetic material in said dissolved carbohydrate so as to obtain a suspension of magnetic material in said dissolved carbohydrate;
- emulsifying said suspension with an emulsion liquid which is immiscible with said suspension so as to form an emulsion containing droplets of said suspension in said emulsion liquid;
- stabilizing said emulsion by contacting said emulsion with a crystallizing liquid different than said polar solvent to thereby crystallize said carbohydrate polymer so as to enclose said magnetic material in spheres of said carbohydrate polymer having an average diameter of less than 1,000 nm;
- and separating said spheres therefrom, said crystallizing liquid being selected from the group consisting of ethanol, methanol, acetone and derivatives thereof.
Dependencies are inferred from the claim text.