Synthesis of genetic females and their use in hybrid seed production
AssigneeRutgers, The State University of New Jersey
InventorOved Shifriss
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Abstract
A synthetic method is described for the development of genetic females in squash (Cucurbita pepo L.) through crosses of monoecious varieties or inbreds followed by selection. The genetic females and true-breeding female lines can be propagated sexually following chemical induction of male flowers. Such female lines can be used as seed parents in production of hybrids as well as germplasm for future breeding programs. The new synthetic method is applicable to other monoecious species of plants.
Claims59
A process for synthesizing true-breeding female lines of squash comprising:
- (a) selecting one monocious variety or inbred as seed parent (P.sub.1) and another monoecious variety or inbred as the pollen parent (P.sub.2), at least one of which is strongly female, said parents being different in breeding history and in genetic makeup;
- (b) crossing P.sub.1 and P.sub.2 ;
- (c) growing F.sub.1 generation from the seed resulting from step (b);
- (d) self pollination of the F.sub.1 generation;
- (e) growing F.sub.2 generation plants from the seeds resulting from step (d);
- (f) selecting an F.sub.2 segregate which has very strong female expression or is female;
- and
- (g) developing a true-breeding female line from said segregate through selection and self-pollination in subsequent filial generation.
A process for synthesizing true-breeding female lines of the Curcurbitaceae family comprising:
- (a) selecting one monoecious variety or inbred as seed parent (P.sub.1) and another monoecious variety or inbred as pollen parent (P.sub.2), at least one of which is strongly female, said parents being different in breeding history and in genetic makeup:
- (b) crossing P.sub.1 and P.sub.2 ;
- (c) growing F.sub.1 generation from the seed resulting from step (b);
- (d) self-pollinating of F.sub.1 generation;
- (e) growing F.sub.2 generation plants from the seeds resulting from step (d);
- (f) selecting an F.sub.2 segregate which has very strong female expression or is female;
- and
- (g) developing a true-breeding female line from said segregate through selection and self-pollination in subsequent filial generations.
A process for synthesizing true-breeding female lines in monoecious species of crop plants comprising:
- (a) selecting one monoecious variety or inbred as seed parent (P.sub.1) and another monoecious variety or inbred as pollen parent (P2), at least one of which is strongly female, said parents being different in breeding history and in genetic makeup.
- (b) crossing P.sub.1 and P.sub.2 ;
- (c) growing F.sub.1 generation from the seed resulting from step (b);
- (d) self-pollinating of F.sub.1 generation;
- (e) growing F.sub.2 generation plants from the seeds resulting from step (b);
- (f) selecting an F.sub.2 segregate which has very strong female expression or is female;
- and
- (g) developing a true-breeding female line from said segregate through selection and self-pollination in subsequent filial generations.
A method of producing hybrid squash seed using a true-breeding female line as seed parent in pollinating proximity to another line of squash which serves as pollen parent and harvesting the seed.
Hybrid squash seed produced by using a true-breeding female line as seed parent in pollinating proximity to another line of squash which served as pollen parent.
A first generation squash plant, the plant having been grown from the seed resulting from crossing a true-breeding female line as seed parent in pollinating proximity to another line of squash which serves as pollen parent.
Squash seed which when grown yields a true-breeding female line.
A true-breeding female line of squash selected from the following species of the botannical genus Cucurbita:
- C. ficifolia Bouche, C. maxima Duch., C. mixta Pang., C. moschata Poir, and C. peno L.
A process for synthesizing a true-breeding female line of squash comprising:
- (a) selecting an existing true-breeding female line as seed parent (P.sub.1) and another line as pollen parent (P.sub.2);
- (b) crossing P.sub.1 and P.sub.2 ;
- (c) growing F.sub.1 generation from the seed resulting from step (b);
- (d) self-pollination of the F.sub.1 generation;
- (e) growing F.sub.2 generation plants from the seeds resulting from step (d);
- (f) selecting an F.sub.2 segregate which has intense female expression or is female and which has one or more improved horticultural characteristics;
- and
- (g) developing a true-breeding female line having the one or more improved horticultural characteristics, from said segregate through selection and self-pollination in subsequent filial generations.
A process for synthesizing new monoecious varieties of squash comprising:
- (a) selecting an existing true-breeding female line as seed parent (P.sub.1) and a monoecious variety as pollen parent (P.sub.2);
- (b) crossing P.sub.1 and P.sub.2 ;
- (c) growing F.sub.1 generation from the seed resulting from step (b);
- (d) self-pollination of the F.sub.1 generation;
- (e) growing F.sub.2 generation plants from the seeds resulting from step (d);
- (f) selecting an F.sub.2 monoecious segregate having one or more improved horticultural characteristics;
- and
- (g) developing a monoecious variety having the one or more improved horticultural characteristics from said segregate through selection and self-pollination in subsequent filial generations.
Dependencies are inferred from the claim text.