Recombinant vector and eukaryotic host transformed thereby
AssigneeWisconsin Alumni Research Foundation
InventorWilliam M. Sugden
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Abstract
A recombinant vector and a eukaryotic host transformed thereby are disclosed. In one embodiment, there is a eukaryotic host that has a recombinant plasmid comprising a plasmid backbone, a foreign eukaryotic gene component, and a first gene segment from a lymphotrophic herpes virus. There is also provided in the host a second gene segment from a lymphotrophic herpes virus. The first and second gene segments interact to assist the plasmid in maintaining itself as a plasmid when inserted into a eukaryotic host, and permit stable replication of the host. The preferred virus is the Epstein-Barr virus, and the two preferred viral segments are the EBV origin of replication and the segment coding for EBNA protein.
Claims8
A recombinant plasmid, comprising:
- a segment from a first plasmid which is not a lymphotrophic herpes virus segment and which facilitates the replication of the recombinant plasmid in a prokaryotic host;
- a segment from a lymphotrohic herpes virus which is linked to said first plasmid segment such that it is capable of assisting in maintaining the recombinant plasmid as a plasmid if the recombinant plasmid is inserted into a eukaryotic host that has been transformed by the lymphotrophic herpes virus;
- and
- a foreign eukaryotic gene component linked as part of the recombinant plasmid.
A eukaryotic host comprising:
- a recombinant plasmid having a segment from a first plasmid which is not a lymphotrophic herpes virus and which facilitates replication of the recombinant plasmid in a prokaryotic host, a segment from a lymphotrophic herpes virus which is linked to said first plasmid segment such that it is capable of assisting in maintaining the recombinant plasmid as a plasmid if the eukaryotic host has been transformed by the lymphotrophic herpes virus, and a foreign eukaryotic gene component linked as part of the recombinant plasmid;
- and
- a second gene segment from the lymphotrophic herpes virus which is not linked to the recombinant plasmid, said second lymphotrophic herpes virus gene segment normally acting in trans to the first lymphotrophic herpes virus gene segment in the naturally occurring virus, whereby both the first and second lymphotrophic herpes virus gene segments assist the recombinant plasmid in maintaining itself as a plasmid.
Dependencies are inferred from the claim text.