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US 4,683,293 AGranted
Purification of pichia produced lipophilic proteins
AssigneePhillips Petroleum Company
InventorWilliam S. Craig
Life of this patent
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Abstract
Method for the selective extraction of desired lipophilic proteins from transformed cells of the genus Pichia by cell lysis in the presence of chaotrophic salts is disclosed. The total protein extracted under the invention cell lysis conditions is reduced while the recovery of desired lipophilic proteins remains relatively constant, thereby producing a cell extract with enhanced concentration of the desired lipophilic protein relative to a control cell extract.
Claims9
A method for the extraction of lipophilic proteins from host cells of the genus Pichia which comprises
- (a) subjecting the cells to cell breaking conditions for a time sufficient to cause breakage of substantially all of the cells, wherein said breakage is carried out in the presence of an extraction medium comprising
- in the range of about 1 up to 8 molar concentration of at least one chaotropic salt, in a medium buffered at a pH suitable to maintain said lipophilic protein in a stable form, and
- (b) recovering the soluble fraction obtained from step (a).
Dependencies are inferred from the claim text.