Novel microorganism and method
AssigneeAustgen-Biojet International Pty. Limited
InventorDavid E. Tribe
Life of this patent
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Abstract
A method for producing phenylalanine comprising culturing novel strains of the bacteria Escherichia coli. The novel strains of E. coli are those in which inhibition of the enzyme DAHP synthease by phenylalanine, tyrosine or tryptophan is removed and inhibition of the enzyme chorismate mutase P-prephenate dehydratase by phenylalanine is removed and in which enhanced levels of production of the enzymes DAHP synthease, chorismate mutase P-prephenate dehydratase and shikimate kinase are achieved. The organism is preferably also modified to limit the production of alternate end products from chorismate.
Claims12
A strain of Escherichia coli having altered production of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase and chorismate mutase P-prephenate dehydratase (CMP-PDH), wherein
- (i) said DAHP synthase is not subject to feedback inhibition by phenylalanine, tryosine, or tryptophan present within the microorganism and said DAHP synthase either
- (a) is not subject to repression by phenylalanine, tyrosine, or tryptophan or
- (b) is produced in greater amount than is produced by wild type E. coli and
- (ii) said CMP-PDH is not subject to feedback inhibition by phenylalanine and said CMP-PDH either
- (a) is not subject to repression by phenylalanine or
- (b) is produced in a greater amount than produced by wild type E. coli.
A method for the production of phenylalanine comprising the steps of culturing a strain of E. coli having altered production of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase and chorismate mutase P-prephenate dehydratase (CMP-PDH), wherein
- (i) said DAHP synthase is not subject to feedback inhibition of phenylalanine, tryosine, or tryptophan present within the microorganism and said DAHP synthase either
- (a) is not subject to repression by phenylalanine, tyrosine, or tryptophan or
- (b) is produced in greater amount than is produced by wild type E. coli and
- (ii) said CMP-PDH is not subject to feedback inhibition by phenylalanine and said CMP-PDH either
- (a) is not subject to repression by phenylalanine or
- (b) is produced in a greater amount than produced by wild type E. coli;
- in a media containing a carbohydrate and recovering the phenylalanine.
Dependencies are inferred from the claim text.