Human tumor necrosis factor
AssigneeCetus Corporation
InventorsDavid F. Mark, Alice M. Wang, Martha B. Ladner, Abla A. Creasey, Janelle N. Van Arsdell, Leo S. Lin
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Abstract
Human Tumor necrosis factor (TNF) has been prepared using recombinant methods. A human promyelocytic leukemia cell line has been induced using an improved induction procedure, and the TNF purified to homogeneity. Methods, vectors, and cells useful in obtaining human TNF in practical amounts are disclosed. Muteins having N-terminal deletions, which muteins have superior biological activity, are also disclosed.
Claims14
Human tumor necrosis factor (TNF) which is .gradient.4-mTNF.
A recombinant DNA sequence which encodes .gradient.4-mTNF.
A recombinant DNA having a DNA sequence encoding .gradient.4-mTNF, operably linked to appropriate regulatory control sequences which are capable of effecting the expression of said coding sequences in transformed cells.
A method for producing human .gradient.4-mTNF which comprises culturing recombinant host cells wherein a recombinant DNA transformed in said host cells has a DNA sequence encoding .gradient.4-mTNF, operably linked to appropriate regulatory control sequence which are capable of effecting the expression of said coding sequences in said transformed cells.
A recombinant vector capable of expression in procaryotic cells which comprises a DNA sequence encoding .gradient.4-mTNF operably linked to control sequences compatible with said procaryotic cells.
Dependencies are inferred from the claim text.