US 4,676,897 AGranted

Solubilization chromatography

AssigneeYokogawa Hokushin Electric Corporation

InventorsShuichi Kuze, Tamotsu Inomata, Setsuo Muramoto, Hisayuki Ikeda

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Abstract

In an apparatus for conducting solubilization chromatography wherein a solvent consisting of a buffer and ionized micells is allowed to flow through a capillary tube whose both ends are connected to the plus and minus sides, respectively, of a DC source, then a sample is introduced into the capillary tube, components of the sample are separated by combination of dissolution phenomenon of the sample in the ionized micells and capillary electro-phoresis, and qualitative and quantitative analysis of the components is carried out; a three way joint is provided having a first opening connected to the capillary tube, a second opening connected to tubing for feeding the solvent and sample, and a third opening serving as a discharge outlet for residue solution of solvent and sample not introduced into the capillary tube; and means are provided for controlling the time the sample contacts an inlet of the capillary tube within the three way joint, whereby the quantity of sample introduced into the capillary tube is controlled. In another embodiment, means are provided for controlling the quantity of sample which is drawn into the capillary tube.

Claims8

Dependent claims

An apparatus for performing solubilization chormatography, comprising

capillary tube having two ends and with one of said ends serving as an inlet;
direct current source having a positive side and a negative side, one end of said capillary tube being disposed on said positive side, and the other end being disposed on the negative side;
means for supplying to said capillary tube a solvent comprising a buffer solution and ionized micells;
means for introducing a sample into said capillary tube, whereby components of said sample are separated by the combined effect of dissolution phenomenon of said sample in said ionized micells and capillary electro-phoresis;
tubing connected to said means for suppying and said means for introducing;
three way joint comprising first, second and third openings, said first opening being connected to said inlet of said capillary tube, said second opening being connected to said tubing to supply said solvent and said sample, and said third opening serving as a discharge outlet for any residual solution of solvent and sample not introduced into said capillary tube, wherein within said three way joint a stream of said solution comprising said sample and said solvent is brought into contact with said inlet of said capillary tube;
means for controlling the contact time of said sample with said inlet of said capillary tube thereby to control the quantity of said sample introduced into said capillary tube;
and
means for qualitative and quantitative analysis of said components of said sample.

6 more elements

An apparatus for performing solubilization chromatography, comprising

capillary tube having two ends and with one of said ends serving as an inlet;
direct current source having a positive side and a negative side, one end of said capillary tube being disposed on said positive side, and the other end being disposed on the negative side;
means for supplying to said capillary tube a solvent comprising a buffer solution and ionized micells;
means for introducing a sample into said capillary tube, whereby components of said sample are separated by the combined effect of dissolution phenomenon of said sample in said ionized micells and capillary electro-phoresis;
tubing connected to said means for supplying and said means for introducing;
three way joint comprising first, second and third openings, said first opening being connected to said inlet of said capillary tube, said second opening being connected to said tubing to supply said solvent and said sample, and said third opening serving as a discharge outlet for any residual solution of said sample and said solvent which is not introduced into said capillary tube, wherein within said three way joint a stream of said solution comprising said sample and said solvent is brought into contact with said inlet of said capillary tube;
means for controlling the quantity of sample drawn into said inlet of said capillary tube;
and
means for qualitative and quantitative analysis of said components of said sample.

6 more elements

Dependencies are inferred from the claim text.

Facts

Application: 06/906,146Filed 11 Sep 1986
Priority: JP 60-21333726 Sep 1985
Granted: 30 Jun 1987
Term ends: Not estimatedThe source records don’t support an estimate.

Classification7 codes

G01N 27/447
Systems: using electrophoresis
B01D 15/3842
Involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or…
G01N 30/58
Conditioning of the sorbent material or stationary liquid: the sorbent moving as a whole
G01N 30/02
Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g.…:…