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US 4,671,954 AGranted
Microspheres for incorporation of therapeutic substances and methods of preparation thereof
AssigneeUniversity of Florida
InventorsEugene P. Goldberg, William E. Longo, Hiroo Iwata
Life of this patent
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Abstract
Novel hydrophilic protein or polypeptide microspheres prepared by dispersing an aqueous solution or dispersion of protein or polypeptide in an organic solvent solution of a high molecular weight polymer to form a stabilized dispersion of microspheres and cross-linking said microspheres with a polyfunctional cross-linking agent.
Claims30
A method of preparing novel hydrophilic microspheres consisting essentially of cross-linked protein or polypeptide comprising
- (a) providing a dispersion of an aqueous solution or dispersion of polypeptide or protein microspheres in an organic, substantially water immiscible solvent solution of a high molecular weight polymer, said organic solvent being substantially a non-solvent for said microspheres and said polymer solution stabilizing the dispersion of microspheres,
- (b) incorporating a polyfunctional cross-linking agent for said protein or polypeptide in the organic phase of said dispersion, and
- (c) allowing said cross-linking agent to react with said protein or polypeptide microspheres for a time sufficient to cross-link at least a portion of the microspheres, thereby providing microspheres containing free reactive functional groups.
Dependencies are inferred from the claim text.