Process for labeling amines with .sup.99m Tc
AssigneeTrustees of the University of Massachusetts
InventorDonald Hnatowich
Life of this patent
Estimating the term…
Abstract
Amines, including proteins and polypeptides covalently coupled with a chelating agent such as diethylenetriaminepentaacetic acid (DTPA) can be labeled with .sup.99m Tc in the presence of a stannous reducing agent at near neutral pH by maintaining the protein concentration at a low level and the DTPA concentration at a high level. The .sup.99m Tc is labeled to the protein through the DTPA sites linked to the protein. Utilizing .sup.111 In labeled proteins as controls, it is shown that the .sup.99m Tc labeled proteins are labeled at the DTPA sites and that they exhibit in vivo stabilities equal to that of the .sup.111 In labeled proteins.
Claims5
A process for making a technetium-99m labeled composition comprising the steps of:
- preparing a chelating agent having the formula ##STR3## wherein R is a linking moiety comprising between 1 and 25 carbon atoms;
- mixing said chelating agent with a host composition selected from the group consisting of an amine, a protein and a polypeptide to covalently link the chelating agent to the host composition, at a ratio of chelating agent to post composition to effect Preferential binding of the technetium-99m to the chelating agent coupled to the host composition while preventing denaturation of the host composition;
- mixing the said host composition covalently linked with said chelating agent with stannous tin at a mole ratio of chelating agent to stannous tin of less than about 1 and at a pH between 6 and 7;
- and
- mixing technetium-99m with said covalently linked post composition and stannous tin.
Dependencies are inferred from the claim text.